![]() We developed packages for high-speed video acquisition, presentation of a visual stimulus library, high-resolution behavioral tracking, and analysis. Furthermore, BonZeb supplies a range of novel utilities that extend Bonsai’s capabilities (Fig. Here, we present BonZeb, an open-source, modular software package developed entirely in Bonsai for high-resolution zebrafish behavioral tracking with virtual open-loop and closed-loop visual stimulation.īonZeb provides an open-source and approachable method to implement the functionalities of extant behavioral feedback systems. Currently, there are no released Bonsai packages that allow for high-speed kinematic tracking of small model organisms, such as the larval zebrafish, while providing behavior based stimulus feedback. Bonsai, an open-source visual programming language, has recently gained traction among the neuroscience community as a powerful programming language designed for the rapid acquisition and processing of multiple data streams 7. Relatively few software implementations have succeeded in addressing this synchronization problem and most rely on software configurations that require advanced levels of technical expertise. A key challenge in developing behavioral feedback systems is synchronizing high-throughput devices. These assays can also increase the throughput of behavioral experiments through the rapid and repeatable delivery of stimuli, and allow researchers to probe the sensorimotor loop by controlling and perturbing sensory feedback 4, 5, 6.ĭespite its significance, the use of behavioral feedback technology is not standard across laboratories investigating sensorimotor integration. Methods for high-resolution behavioral tracking and dynamic sensory feedback can be combined with methods for monitoring or manipulating neural circuit activity to allow researchers to explore the neural circuitry underlying sensorimotor behaviors. The combination of precision tracking with systems for behavioral feedback stimulus delivery can provide valuable insights into the relationship between sensory inputs and behavioral outputs 1, 2, 3. The ability to precisely track animal movements is vital to the goal of relating the activity of the nervous system to behavior.
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